With Argentina and Colombia already confirmed to play in Brazil, two spots are available for three sides fighting it out.
With Uruguay losing to Ecuador on Friday, it means both Reinaldo Rueda's side and Chile need just one point from this match to make it impossible for Uruguay, who sit in fifth, to leapfrog them.
Chile come into this match unbeaten in their last eight games. Jorge Sampaoli's side had started qualification in fine form, winning four of their first six matches.
However, a poor run at the back end of last year saw them lose four in a row to put their qualification spot in jeopardy.
Sampaoli's men then picked up their form again to win four of their last five matches, which puts them in pole position to qualify for Brazil. Bet365 price them at 7/5 (2.40) to win.
